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KMID : 1134820110400121787
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 12 p.1787 ~ p.1792
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Comparison of Enrichment Media of Shigella sonnei
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In Ye-Won
Ha Su-Jeong
Kim Seok-Joong
Oh Se-Wook
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Abstract
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The object of this study was to compare the performance of commercial enrichment media used for Shigella spp. A total of four enrichment media, Gram negative (GN) broth, Shigella broth (SB), selenite-F (SF) broth, and selenite cystine (SC) broth, were tested. When S. sonnei was inoculated into each enrichment broth at 10 cfu/mL of concentration, the highest growth was observed in Shigella broth. Morganella spp., which was not differentiated in selective agar of Shigella spp. thus can be counted as false positive, did not grow in Shigella broth in enrichment step. When S. sonnei was artificially inoculated into pork, it was mostly recovered through an enrichment process with GN broth and SF broth. However, in the case of beef, S. sonnei was mostly recovered with GN broth but largely failed with Shigella broth. Therefore, enrichment media for Shigella spp. should be selected by considering the food matrix in order to increase the chance of isolating it from foods.
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KEYWORD
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Shigella sonnei, enrichment broth, recovery, performance
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